envelope n term (ProSci Incorporated)
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Envelope N Term, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+n+term/SARS-CoV-2+(COVID-19)+Envelope+Antibody/bio_rxiv__2025__03__29__646083-207-57-59
Average 90 stars, based on 7 article reviews
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Staining:Article Title: Ketamine inhibition of SARS-CoV-2 replication in astrocytes is associated with COVID-19 disease severity in a variant-dependent manner Article Snippet: After fixation and permeabilization cells were washed with PBS and incubated in 10% goat serum (Sigma-Aldrich G6767) and 3% bovine serum albumin (BSA; Sigma-Aldrich, A2153) solution in PBS for 1 h at 37°C to reduce non-specific binding of antibodies. .. Non-specific background staining was reduced by incubating the cells in a blocking buffer containing 3% BSA and 10% goat serum in PBS at 37 °C for 1 h. After the blocking step cells were incubated with primary antibodies against angiotensin-converting-enzyme 2 (Abcam, ab89111, dilution 1:50) and against two SARS-CoV-2 antigens: RNA-dependent-RNA-polymerase nsp12 (Genetex, GTX135467, dilution 1:100) or Blocking Assay:Article Title: Ketamine inhibition of SARS-CoV-2 replication in astrocytes is associated with COVID-19 disease severity in a variant-dependent manner Article Snippet: After fixation and permeabilization cells were washed with PBS and incubated in 10% goat serum (Sigma-Aldrich G6767) and 3% bovine serum albumin (BSA; Sigma-Aldrich, A2153) solution in PBS for 1 h at 37°C to reduce non-specific binding of antibodies. .. Non-specific background staining was reduced by incubating the cells in a blocking buffer containing 3% BSA and 10% goat serum in PBS at 37 °C for 1 h. After the blocking step cells were incubated with primary antibodies against angiotensin-converting-enzyme 2 (Abcam, ab89111, dilution 1:50) and against two SARS-CoV-2 antigens: RNA-dependent-RNA-polymerase nsp12 (Genetex, GTX135467, dilution 1:100) or Incubation:Article Title: Ketamine inhibition of SARS-CoV-2 replication in astrocytes is associated with COVID-19 disease severity in a variant-dependent manner Article Snippet: After fixation and permeabilization cells were washed with PBS and incubated in 10% goat serum (Sigma-Aldrich G6767) and 3% bovine serum albumin (BSA; Sigma-Aldrich, A2153) solution in PBS for 1 h at 37°C to reduce non-specific binding of antibodies. .. Non-specific background staining was reduced by incubating the cells in a blocking buffer containing 3% BSA and 10% goat serum in PBS at 37 °C for 1 h. After the blocking step cells were incubated with primary antibodies against angiotensin-converting-enzyme 2 (Abcam, ab89111, dilution 1:50) and against two SARS-CoV-2 antigens: RNA-dependent-RNA-polymerase nsp12 (Genetex, GTX135467, dilution 1:100) or |

