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envelope n term  (ProSci Incorporated)


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    Structured Review

    ProSci Incorporated envelope n term
    Envelope N Term, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/envelope+n+term/SARS-CoV-2+(COVID-19)+Envelope+Antibody/bio_rxiv__2025__03__29__646083-207-57-59
    Average 90 stars, based on 7 article reviews
    envelope n term - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Ketamine inhibition of SARS-CoV-2 replication in astrocytes is associated with COVID-19 disease severity in a variant-dependent manner
    Article Snippet: After fixation and permeabilization cells were washed with PBS and incubated in 10% goat serum (Sigma-Aldrich G6767) and 3% bovine serum albumin (BSA; Sigma-Aldrich, A2153) solution in PBS for 1 h at 37°C to reduce non-specific binding of antibodies. .. Non-specific background staining was reduced by incubating the cells in a blocking buffer containing 3% BSA and 10% goat serum in PBS at 37 °C for 1 h. After the blocking step cells were incubated with primary antibodies against angiotensin-converting-enzyme 2 (Abcam, ab89111, dilution 1:50) and against two SARS-CoV-2 antigens: RNA-dependent-RNA-polymerase nsp12 (Genetex, GTX135467, dilution 1:100) or envelope N-term (ProSci, 3531, dilution 1:100) for 2 h at 37 °C or overnight at 4 °C. .. The cells were then rinsed in PBS and incubated in secondary antibodies against mouse or rabbit IgGs coupled to Alexa Fluor 546 (Invitrogen, Thermo Fisher Scientific, A110033; dilution 1:600) and Alexa Fluor 488 (Invitrogen, Thermo Fisher Scientific, A11008, dilution 1:600) for 45 min at 37°C (the mixture of secondary antibodies was applied simultaneously).

    Blocking Assay:

    Article Title: Ketamine inhibition of SARS-CoV-2 replication in astrocytes is associated with COVID-19 disease severity in a variant-dependent manner
    Article Snippet: After fixation and permeabilization cells were washed with PBS and incubated in 10% goat serum (Sigma-Aldrich G6767) and 3% bovine serum albumin (BSA; Sigma-Aldrich, A2153) solution in PBS for 1 h at 37°C to reduce non-specific binding of antibodies. .. Non-specific background staining was reduced by incubating the cells in a blocking buffer containing 3% BSA and 10% goat serum in PBS at 37 °C for 1 h. After the blocking step cells were incubated with primary antibodies against angiotensin-converting-enzyme 2 (Abcam, ab89111, dilution 1:50) and against two SARS-CoV-2 antigens: RNA-dependent-RNA-polymerase nsp12 (Genetex, GTX135467, dilution 1:100) or envelope N-term (ProSci, 3531, dilution 1:100) for 2 h at 37 °C or overnight at 4 °C. .. The cells were then rinsed in PBS and incubated in secondary antibodies against mouse or rabbit IgGs coupled to Alexa Fluor 546 (Invitrogen, Thermo Fisher Scientific, A110033; dilution 1:600) and Alexa Fluor 488 (Invitrogen, Thermo Fisher Scientific, A11008, dilution 1:600) for 45 min at 37°C (the mixture of secondary antibodies was applied simultaneously).

    Incubation:

    Article Title: Ketamine inhibition of SARS-CoV-2 replication in astrocytes is associated with COVID-19 disease severity in a variant-dependent manner
    Article Snippet: After fixation and permeabilization cells were washed with PBS and incubated in 10% goat serum (Sigma-Aldrich G6767) and 3% bovine serum albumin (BSA; Sigma-Aldrich, A2153) solution in PBS for 1 h at 37°C to reduce non-specific binding of antibodies. .. Non-specific background staining was reduced by incubating the cells in a blocking buffer containing 3% BSA and 10% goat serum in PBS at 37 °C for 1 h. After the blocking step cells were incubated with primary antibodies against angiotensin-converting-enzyme 2 (Abcam, ab89111, dilution 1:50) and against two SARS-CoV-2 antigens: RNA-dependent-RNA-polymerase nsp12 (Genetex, GTX135467, dilution 1:100) or envelope N-term (ProSci, 3531, dilution 1:100) for 2 h at 37 °C or overnight at 4 °C. .. The cells were then rinsed in PBS and incubated in secondary antibodies against mouse or rabbit IgGs coupled to Alexa Fluor 546 (Invitrogen, Thermo Fisher Scientific, A110033; dilution 1:600) and Alexa Fluor 488 (Invitrogen, Thermo Fisher Scientific, A11008, dilution 1:600) for 45 min at 37°C (the mixture of secondary antibodies was applied simultaneously).



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    JGF inhibits NO, IL-6, and TNF-α production in RAW264.7 and MH-S cells. The cells were treated with JGF (50, 100, 150, 300, 600 μg/mL), 2-E (0.1 μM), DXT (10 μM), or LPS (0.1 μg/mL) for 24 h. ( A ) Cell viability was evaluated using crystal violet. ( B ) NO production was measured using the Griess assay. ( C-D ) IL-6 ( C ) and TNF-α ( D ) levels were determined by ELISA. EC 50 was calculated by CompuSyn software. Data was presented as mean ± standard deviation (SD) for groups (n = 3). Significant differences are denoted as ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Chemical characterization of Jing Guan Fang and its application in alleviating coronavirus envelope protein-induced proinflammatory responses in vitro and in vivo

    doi: 10.1016/j.jtcme.2025.12.003

    Figure Lengend Snippet: JGF inhibits NO, IL-6, and TNF-α production in RAW264.7 and MH-S cells. The cells were treated with JGF (50, 100, 150, 300, 600 μg/mL), 2-E (0.1 μM), DXT (10 μM), or LPS (0.1 μg/mL) for 24 h. ( A ) Cell viability was evaluated using crystal violet. ( B ) NO production was measured using the Griess assay. ( C-D ) IL-6 ( C ) and TNF-α ( D ) levels were determined by ELISA. EC 50 was calculated by CompuSyn software. Data was presented as mean ± standard deviation (SD) for groups (n = 3). Significant differences are denoted as ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Article Snippet: SARS-CoV-2-envelope (2-E) protein (Cat# NBP2–90986) was purchased from Novus Biologicals (Littleton, CO, USA).

    Techniques: Griess Assay, Enzyme-linked Immunosorbent Assay, Software, Standard Deviation

    Components of JGF inhibit 2-E-induced inflammation. The RAW264.7 and MH-S cells were co-treated with JGF compounds and 2-E for 24 h. ( A ) The 3D-HPLC fingerprint of JGF. Compound structures were sourced from the PubChem database. The detection wavelength ranged from 200 to 400 nm, and the injection volume was 20 μL. ( B ) Cell viability was evaluated using crystal violet. ( C ) NO production was measured using the Griess assay. ( D-E ) IL-6 ( D ) and TNF-α ( E ) levels were determined by ELISA. Data are presented as mean ± SD (n = 3). Statistical significance was determined relative to the 2-E group. Significant differences are denoted as ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Chemical characterization of Jing Guan Fang and its application in alleviating coronavirus envelope protein-induced proinflammatory responses in vitro and in vivo

    doi: 10.1016/j.jtcme.2025.12.003

    Figure Lengend Snippet: Components of JGF inhibit 2-E-induced inflammation. The RAW264.7 and MH-S cells were co-treated with JGF compounds and 2-E for 24 h. ( A ) The 3D-HPLC fingerprint of JGF. Compound structures were sourced from the PubChem database. The detection wavelength ranged from 200 to 400 nm, and the injection volume was 20 μL. ( B ) Cell viability was evaluated using crystal violet. ( C ) NO production was measured using the Griess assay. ( D-E ) IL-6 ( D ) and TNF-α ( E ) levels were determined by ELISA. Data are presented as mean ± SD (n = 3). Statistical significance was determined relative to the 2-E group. Significant differences are denoted as ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Article Snippet: SARS-CoV-2-envelope (2-E) protein (Cat# NBP2–90986) was purchased from Novus Biologicals (Littleton, CO, USA).

    Techniques: Injection, Griess Assay, Enzyme-linked Immunosorbent Assay

    JGF downregulates 2-E-induced iNOS and COX-2 in RAW264.7 and MH-S cells. Cells were treated with JGF (0, 50, 200 μg/mL) or 2-E (0.1 μM) for 24 h. ( A ) Protein levels of iNOS and COX-2 in macrophages were measured by Western blot. ( B-C ) Quantification of iNOS and COX-2 in cells without ( B ) and with ( C ) 2-E stimulation, calculated using ImageJ. Actin was used as the internal control. The non-detected data showed as – or ND. Data are presented as mean ± SD (n = 3). Significant differences are denoted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Chemical characterization of Jing Guan Fang and its application in alleviating coronavirus envelope protein-induced proinflammatory responses in vitro and in vivo

    doi: 10.1016/j.jtcme.2025.12.003

    Figure Lengend Snippet: JGF downregulates 2-E-induced iNOS and COX-2 in RAW264.7 and MH-S cells. Cells were treated with JGF (0, 50, 200 μg/mL) or 2-E (0.1 μM) for 24 h. ( A ) Protein levels of iNOS and COX-2 in macrophages were measured by Western blot. ( B-C ) Quantification of iNOS and COX-2 in cells without ( B ) and with ( C ) 2-E stimulation, calculated using ImageJ. Actin was used as the internal control. The non-detected data showed as – or ND. Data are presented as mean ± SD (n = 3). Significant differences are denoted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: SARS-CoV-2-envelope (2-E) protein (Cat# NBP2–90986) was purchased from Novus Biologicals (Littleton, CO, USA).

    Techniques: Western Blot, Control

    JGF inhibits 2-E-induced phosphorylation of STAT3 in RAW264.7 and MH-S cells. Cells were treated with JGF (0, 50, 200 μg/mL) or 2-E (0.1 μM) for 3 h. ( A ) Protein levels of phosphorylated JAK2 and STAT3 were measured by Western blot. ( B-C ) Quantification of phosphorylated JAK2 and STAT3 in cells without ( B ) and with ( C ) 2-E stimulation, calculated using ImageJ. Actin was used as the internal control. Data are presented as mean ± SD (n = 3). Significant differences are denoted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Chemical characterization of Jing Guan Fang and its application in alleviating coronavirus envelope protein-induced proinflammatory responses in vitro and in vivo

    doi: 10.1016/j.jtcme.2025.12.003

    Figure Lengend Snippet: JGF inhibits 2-E-induced phosphorylation of STAT3 in RAW264.7 and MH-S cells. Cells were treated with JGF (0, 50, 200 μg/mL) or 2-E (0.1 μM) for 3 h. ( A ) Protein levels of phosphorylated JAK2 and STAT3 were measured by Western blot. ( B-C ) Quantification of phosphorylated JAK2 and STAT3 in cells without ( B ) and with ( C ) 2-E stimulation, calculated using ImageJ. Actin was used as the internal control. Data are presented as mean ± SD (n = 3). Significant differences are denoted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: SARS-CoV-2-envelope (2-E) protein (Cat# NBP2–90986) was purchased from Novus Biologicals (Littleton, CO, USA).

    Techniques: Phospho-proteomics, Western Blot, Control

    JGF inhibits 2-E-induced phosphorylation of ERK1/2 in RAW264.7 and MH-S cells. Cells were treated with JGF (0, 50, 200 μg/mL) or 2-E (0.1 μM) for 3 h. ( A ) Protein levels of phosphorylated JNK1/2, ERK1/2, p38, and p65 were measured by Western blot. ( B-C ) Quantification of phosphorylated JNK1/2, ERK1/2, p38, and p65 in cells without ( B ) and with ( C ) 2-E stimulation, calculated using ImageJ. Actin was used as the internal control. Data are presented as mean ± SD (n = 3). Significant differences are denoted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Chemical characterization of Jing Guan Fang and its application in alleviating coronavirus envelope protein-induced proinflammatory responses in vitro and in vivo

    doi: 10.1016/j.jtcme.2025.12.003

    Figure Lengend Snippet: JGF inhibits 2-E-induced phosphorylation of ERK1/2 in RAW264.7 and MH-S cells. Cells were treated with JGF (0, 50, 200 μg/mL) or 2-E (0.1 μM) for 3 h. ( A ) Protein levels of phosphorylated JNK1/2, ERK1/2, p38, and p65 were measured by Western blot. ( B-C ) Quantification of phosphorylated JNK1/2, ERK1/2, p38, and p65 in cells without ( B ) and with ( C ) 2-E stimulation, calculated using ImageJ. Actin was used as the internal control. Data are presented as mean ± SD (n = 3). Significant differences are denoted as ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: SARS-CoV-2-envelope (2-E) protein (Cat# NBP2–90986) was purchased from Novus Biologicals (Littleton, CO, USA).

    Techniques: Phospho-proteomics, Western Blot, Control

    JGF reduces the 2-E-induced proinflammatory cytokines in vivo . ( A ) The experimental scheme for mouse exposure. ( B-F ) Levels of IL-6 ( B ), TNF-α ( C ), IFN-γ ( D ), IL-1β ( E ), and IL-12 ( F ) in lung tissue and serum were measured by ELISA. Data are presented as mean ± SD (n = 9 for serum, except DXT group n = 6; n = 6 for lung tissue, except DXT group n = 3) ( G ) Representative histological images of lung tissue stained with H&E and IHC images for IL-6, TNF-α, and IL-1β expression. ( H-J ) Quantification of IL-6 ( H ), TNF-α ( I ), and IL-1β ( J ) positive areas using ImageJ (n = 3). Significant differences between the control (CTL) group and other groups are denoted by ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Significant differences between the 2-E group and 2-E + JGF group are indicated by #p < 0.05, ##p < 0.01, ###p < 0.001.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Chemical characterization of Jing Guan Fang and its application in alleviating coronavirus envelope protein-induced proinflammatory responses in vitro and in vivo

    doi: 10.1016/j.jtcme.2025.12.003

    Figure Lengend Snippet: JGF reduces the 2-E-induced proinflammatory cytokines in vivo . ( A ) The experimental scheme for mouse exposure. ( B-F ) Levels of IL-6 ( B ), TNF-α ( C ), IFN-γ ( D ), IL-1β ( E ), and IL-12 ( F ) in lung tissue and serum were measured by ELISA. Data are presented as mean ± SD (n = 9 for serum, except DXT group n = 6; n = 6 for lung tissue, except DXT group n = 3) ( G ) Representative histological images of lung tissue stained with H&E and IHC images for IL-6, TNF-α, and IL-1β expression. ( H-J ) Quantification of IL-6 ( H ), TNF-α ( I ), and IL-1β ( J ) positive areas using ImageJ (n = 3). Significant differences between the control (CTL) group and other groups are denoted by ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Significant differences between the 2-E group and 2-E + JGF group are indicated by #p < 0.05, ##p < 0.01, ###p < 0.001.

    Article Snippet: SARS-CoV-2-envelope (2-E) protein (Cat# NBP2–90986) was purchased from Novus Biologicals (Littleton, CO, USA).

    Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Control

    Schematics showing the anti-inflammatory mechanism of JGF in 2-E-induced mice macrophages.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Chemical characterization of Jing Guan Fang and its application in alleviating coronavirus envelope protein-induced proinflammatory responses in vitro and in vivo

    doi: 10.1016/j.jtcme.2025.12.003

    Figure Lengend Snippet: Schematics showing the anti-inflammatory mechanism of JGF in 2-E-induced mice macrophages.

    Article Snippet: SARS-CoV-2-envelope (2-E) protein (Cat# NBP2–90986) was purchased from Novus Biologicals (Littleton, CO, USA).

    Techniques:

    Journal: iScience

    Article Title: SARS-CoV-2 envelope protein regulates innate immune tolerance

    doi: 10.1016/j.isci.2024.109975

    Figure Lengend Snippet:

    Article Snippet: Recombinant SARS-CoV-2 Envelope protein , Novus Biologicals , NBP2-90986.

    Techniques: Recombinant, Selection, Isolation, Software